Development of a Certified Reference Material from Caffeine Solution for Assuring the Quality of Food and Drug Measurements

Caffeine intake by pregnant women, adults and children can be harmful to the health of all particularly fetuses if exceeds permissible limits. Therefore, it is fundamental importance measure its concentration accurately using certified reference materials (CRMs). In literature, no scientific details are published about certification caffeine standard solutions, therefore, present article covers this gap. A batch solution was prepared in 1000 mg/kg bottled. Homogeneity stability candidate material were assessed HPLC-UV results showed that homogenous stable enough. Characterization performed HPLC-UV, LC-MS/MS UV-VIS-NIR spectrophotometer three different days characterization uncertainty estimated accordance with requirements ISO GUM. The value (999.86 ± 8.57 mg/kg) derived as a weighted mean from gravimetry methods calculated according Guide 35. produced CRM strong interest food drug analytical laboratories for validity credibility their measurement results.